Proteomic approaches to profiling the humoral immune response and identifying disease-associated antigens.

Humoral Immune Response of Cultured Persian Sturgeon (Acipenser persicus) to Four Different Aeromonas hydrophila Antigens

Proteomic profiling of antigens in circulating immune complexes associated with each of seven autoimmune diseases.

OBJECTIVE Immune complexes (ICs) trigger humoral immune responses. Therefore, the identification of constituent antigens within ICs would have very different clinical significance than identification of free antigens. DESIGN AND METHODS Here, we applied immune complexome analysis of serum to the study of seven major autoimmune diseases-anti-neutrophil cytoplasmic antibody-associated vasculiti...

Interpretation of Serologic Test Results and Evaluation of Humoral Immune Response After Vaccination Following Infection with COVID-19 Disease

SARS-CoV-2 virus infection induces a cellular and humoral mediated immune response and produces antibodies against viral antigens such as nucleocapsid (N) protein and spike (S) protein. Anti-protein S antibodies also target the spike protein subunit S1 and the receptor binding domain (RBD). Serologic tests can detect the presence of these antibodies in blood serum within a few days to a few wee...

Two-dimensional immune profiles improve antigen microarray-based characterization of humoral immunity.

Antigen arrays are becoming widely used tools for the characterization of the complexity of humoral immune responses. Current antibody profiling techniques provide modest and indirect information about the effector functions of the antibodies that bind to particular antigens. Here we introduce an antigen array-based approach for obtaining immune profiles reflecting antibody functionality. This ...

Proteomic-Based Approach for the Identification of Tumor Markers Associated with Hepatocellular Carcinoma

There is increasing evidence for an immune response to cancer in humans, demonstrated in part by the identification of autoantibodies to tumor antigens. The identification of panels of tumor antigens that elicit a humoral response may have utility in cancer screening, diagnosis or in establishing prognosis. Several approaches are currently available for the identification of tumor antigens. We ...